- Which types of culture media are solid?
- What is a positive slant language?
- What is negative slant language?
- How do you create a slant culture?
- How do you prepare a bacterial stock culture?
- Why are agar slants better for stock cultures?
- How do you maintain stock culture?
- What is the difference between a slant and a broth?
- Why should you always mix up a broth culture before using it for the first time?
- What two tools are most commonly used to transfer bacteria?
- How do the pure broth cultures differ the slant cultures?
- How do you revive ATCC culture?
- Can bacteria grow in glycerin?
- How do you know if a broth culture is pure?
- What is an example of slant?
- What is the purpose of slant culture?
- What is the definition of slant?
- What are slant cultures used for quizlet?
Which types of culture media are solid?
Solid media is used for the isolation of bacteria as pure culture.
‘Agar’ is most commonly used to prepare solid media.
Agar is polysaccharide extract obtained from seaweed..
What is a positive slant language?
What is slanted language? Its the use of language to state your positive/negative opinions about the subject of your statement instead of being neutral. Both sides of the chart above describe similar subjects but also show the opinion of the speaker about the subjects (us vs. them).
What is negative slant language?
I’d like to know the meaning of this phrase “negative slant” as used in this sentence, “If an evil main character wins out over some force of nature, then the story theme will have a negative slant.”
How do you create a slant culture?
Slanting. Agar slants are created by bringing agar to the boiling point and pouring it into a test tube. Before the agar cools and solidifies, the test tube is set on its side. Once the agar is cooled, the test tube can be stored upright, and the agar inside has a slanted appearance.
How do you prepare a bacterial stock culture?
ProcedureFollow the steps for Inoculating an Overnight Liquid Culture.After you have bacterial growth, add 500 μL of the overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix.Freeze the glycerol stock tube at -80°C.More items…
Why are agar slants better for stock cultures?
Why are agar slants better suited than agar plates to maintain stock cultures? Slants are better suited because they can be capped, preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
How do you maintain stock culture?
Preserving Stock Cultures A popular method for preserving microorganisms is through lyophilization, or freeze drying. This method is best for long term storage. The lyophilization process requires costly equipment including a lyophilizer, vacuum pump, generator, etc.
What is the difference between a slant and a broth?
Broth=dip loop into other broth; slant=streak loop across surface (zigzag); deep=use inocul.
Why should you always mix up a broth culture before using it for the first time?
Bacterial liquid cultures should be shaken always (but not too vigorously), in order to ensure aeration and oxygen and nutrient availability as well as to avoid bacterial settlement on the flask bottom which would result in cell death from the lack of nutrient availability.
What two tools are most commonly used to transfer bacteria?
Inoculating loops and needles made of metal wire can be used to transfer bacteria from one medium to another, such as from the surface of an agar plate to a broth. Metal tools may be sterilized by heating them in the flame of a Bunsen burner.
How do the pure broth cultures differ the slant cultures?
How do the pure broth cultures differ? … Broth cultures are in a liquid phase because they lack agar and slant cultures are a solid medium that occupies a test tube at an angle to allow a large surface area for growth.
How do you revive ATCC culture?
Bacteria. For frozen cultures, thaw the bacterial strain using gentle agitation in a water bath that is set to 25°C to 30°C. Thawing will be rapid; approximately 2 minutes or until all ice crystals have melted.
Can bacteria grow in glycerin?
Putting a happy aqueous bacterium into 100% glycerol will cause immense osmotic shock, probably resulting in death for most bacteria, due to too much of their intracellular water trying to escape to the extracellular, water-free (but glycerol rich) environment.
How do you know if a broth culture is pure?
You can determine if a broth culture is pure ( all one species of bacteria) by visually inspecting without a microscope. It is not harmful to the bacteria to use a loop that hasn’t been cooled. A loop is cooled as soon as it is no longer red. Why do you invert the plate during incubation?
What is an example of slant?
The definition of a slant is an incline or a point of view. An example of a slant is an upward slope. An example of a slant is a conservative editorial approach in a newspaper.
What is the purpose of slant culture?
Slanting the surface of the agar gives the bacteria a greater surface area on which to grow in a test tube. Furthermore, slants are created in test tubes that can be capped, which minimizes water loss. This is important because of the high moisture content of agar media.
What is the definition of slant?
1 : to give an oblique or sloping direction to. 2 : to interpret or present in line with a special interest : angle stories slanted toward youth especially : to maliciously or dishonestly distort or falsify. slant. noun. Definition of slant (Entry 2 of 2)
What are slant cultures used for quizlet?
Agar slants are test tubes containing solid media that were left an an angle while the agar solidified. They provide a solid growth surface for bacteria and are also easily stored and transported.